منابع مشابه
Solid friction in gel electrophoresis
We study the influence of solid frictional forces acting on polymer chains moving in a random environment. We show that the total reduction in the chain tension resulting from the small friction between a polymer and fixed entanglement points is a steep nonlinear function of the number of entanglements ~exponential for stretched chains!. Therefore, solid friction can drastically change the dyna...
متن کاملGEL, a DNA sequencing project management system
We have developed an automated system for management of DNA sequencing projects. The system, named GEL, can handle data from both random sequences and from fragments whose relative positions are known. The system is highly interactive, self-documenting, and forgiving; it is designed for use by computer-naive molecular biologists. An editor designed specifically for sequences allows simple entry...
متن کاملDirect sequencing of PCR products in agarose gel slices.
Direct DNA sequencing of amplified polymerase chain reaction (PCR) products offers several advantages over cloning of amplified DNA products. It is faster (1 day versus 3—5 days) and in DNA samples containing sequence polymorphisms both the normal and mutated sequence can be detected in the same sequencing reaction. The major problems encountered in direct sequencing of amplified DNA have been ...
متن کاملRapid DNA sequencing by horizontal ultrathin gel electrophoresis.
A horizontal polyacrylamide gel electrophoresis apparatus has been developed that decreases the time required to separate the DNA fragments produced in enzymatic sequencing reactions. The configuration of this apparatus and the use of circulating coolant directly under the glass plates result in heat exchange that is approximately nine times more efficient than passive thermal transfer methods ...
متن کاملElectrophoresis of unlabeled proteins in a sequencing gel apparatus.
In an earlier issue of this journal, Whelan and Taylor (4) described the adaptation of the Sequi-Gen electrophoretic cell (Bio-Rad Laboratories, Hercules, CA, USA) for analysis of proteins. Their paper demonstrated that, with slight modifications of the plate assembly and gel loading protocols, it was possible to obtain a large degree of separation of the radiolabeled polypeptides electrophore...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 1999
ISSN: 1087-0156,1546-1696
DOI: 10.1038/8562